Sodium butyrate protected the intestinal barrier of hyperuricemia mice and reduced serum uric acid levels by reversing the decreased expression of tight junction proteins.
Does sodium butyrate reduce serum uric acid and protect the intestinal barrier in hyperuricemia models?
Sodium butyrate demonstrates protective effects on the intestinal barrier and reduces serum uric acid levels in preclinical models of hyperuricemia.
The goal of this study was to examine the role of sodium butyrate in preserving the intestinal mucosal barrier and reducing hyperuricemia (HUA). First, we established a mouse model of HUA via intraperitoneal injection of potassium oxonate together with a yeast-rich diet to detect the levels of serum uric acid (UA) and fecal short-chain fatty acids (SCFAs). Then, in vitro, different concentrations of UA and sodium butyrate (NaB) were used to treat LS174T and Caco2 cells. The effects of UA and NaB on the gut barrier were determined based on the expression levels of MUC2, ZO-1, and Occludin.Finally, C57BL/6 mice were used to model HUA, and these mice were administered 200 mg·kg−1·d−1 NaB by gavage to counter the HUA. The effect of NaB on HUA in the intestinal tract was elucidated by determining serum UA levels, inflammatory parameters, epithelial barrier integrity, and via histological analysis. The data showed that the content of fecal SCFAs in HUA mice decreased. Additionally, in LS174T and Caco2 cells, NaB reversed the decrease of ZO-1, Occludin, and MUC2 protein expression caused by high UA levels. Furthermore, NaB decreased serum UA of HUA mice, and reversed both the decreased expression of MUC2, ZO-1, Occludin, and ABCG2 proteins and the increased level of inflammatory factors in the intestinal tissues of these mice. The HUA mouse model showed intestinal barrier damage. NaB protected the intestinal barrier of HUA mice and reduced the serum UA level.
Li et al. (Mon,) conducted a other in Hyperuricemia. Sodium butyrate vs. Hyperuricemia model without sodium butyrate was evaluated on Intestinal mucosal barrier preservation and serum uric acid reduction. Sodium butyrate protected the intestinal barrier of hyperuricemia mice and reduced serum uric acid levels by reversing the decreased expression of tight junction proteins.