Key result
Nucleotide mutations on the stem-loop structure in the junction region of the HEV genome significantly reduced or abolished virus replication in Huh7 cells.
Both the sequence and the stem-loop structure in the junction region of the HEV genome are critical for viral replication.
Disrupting the AGA motif abolishes HEV replication in animal models; hypothesis-generating for antivirals but unproven in humans.
The roles of conserved nucleotides on the stem-loop (SL) structure in the intergenic region of the hepatitis E virus (HEV) genome in virus replication were determined by using Huh7 cells transfected with HEV SL mutant replicons containing reporter genes. One or two nucleotide mutations of the AGA motif on the loop significantly reduced HEV replication, and three or more nucleotide mutations on the loop abolished HEV replication. Mutations on the stem and of the subgenome start sequence also significantly inhibited HEV replication. The results indicated that both the sequence and the SL structure in the junction region play important roles in HEV replication.
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Cao et al. (2010) studied Hepatitis E virus (HEV) replication. Nucleotide mutations on the stem-loop structure of the HEV genome vs. Wild-type HEV replicon was evaluated on HEV replication. Nucleotide mutations on the stem-loop structure in the junction region of the HEV genome significantly reduced or abolished virus replication in Huh7 cells.
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