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In this work, a biological recovery of metals (copper and gold) from computer printed circuit board (PCB) waste was carried out by bioleaching using Aspergillus niger. Three bioleaching methods comprising one or two steps or using spent medium were tested in an incubator shaker at 30 °C and 160 rpm with different PCB waste concentrations (2.5 to 10 g/L). Glucose was used as the carbon source. The best condition evaluated was carried out in a stirred tank reactor. The FTIR spectrum confirmed the presence of oxalic, citric, and gluconic acids. A. niger showed an efficiency of bioleaching of up to 100% and 42.5% for copper and gold, respectively, using the two-step method with 2.5 g/L PCB waste after 14 days of the process. The efficiency of bioleaching in a stirred tank reactor was 83% for copper and 24% for gold. The mean metallic particle size obtained after bioleaching varied according to the PCB waste concentration (2.5–10 g/L) added in the experiments. A transmission electron microscope analysis confirmed the synthesis of metallic nanoparticles with spherical morphology. The results indicated that the PCBs bioleaching process with A. niger can be an environmentally friendly alternative to current mechanical and metallurgical processes for metal leaching.
Rodrigues et al. (Mon,) studied this question.