Chemically fashioned ZnO nanowalls and their potential application for potentiometric cholesterol biosensor

Chemically fashioned zinc oxide (ZnO) nanowalls on aluminum wire have been characterized and utilized to fabricate a potentiometric cholesterol biosensor by an electrostatic conjugation with cholesterol oxidase. The sensitivity, specificity, reusability, and stability of the conjugated surface of ZnO nanowalls with thickness of ∼80 nm have been investigated over a wide logarithmic concentrations of cholesterol electrolyte solution ranging from 1×10−6–1×10−3 M. The presented biosensor illustrates good linear sensitivity slope curve (∼53 mV/decade) corresponding to cholesterol concentrations along with rapid output response time of ∼5 s.