Prospective Evaluation of Genetic Variation in Platelet Endothelial Aggregation Receptor 1 Reveals Aspirin‐Dependent Effects on Platelet Aggregation Pathways

Figures

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  Figure 1. Each study participant underwent 7‐day aspirin interventions at a low (81 mg/day), medium (162 mg/day), and high aspirin dose (324 mg/day), with a minimum 2‐week washout in between each aspirin regimen. Platelet aggregation was measured at day 0 prior to the first aspirin dose and on day 7 following the final aspirin dose of each treatment period. Blood and urine samples were also obtained at day 0 and day 7 of each treatment period. PAPI, Pharmacogenomics of Anti‐Platelet Intervention; PEAR1, platelet endothelial aggregation receptor 1.

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  Figure 2. Baseline agonist‐induced ex vivo platelet aggregation measurements were obtained in platelet‐rich plasma prior to administration of the first aspirin dose. Platelet aggregation is expressed as the percentage of maximal aggregation using platelet‐poor plasma as a referent. Adenosine diphosphate (ADP; 2 μM), collagen (2 μg/mL), epinephrine (10 μM), and arachidonic acid (AA; 1.6 mM) were used to stimulate platelet aggregation. The horizontal line within each box indicates the median; the top and bottom borders of each box indicate the interquartile range (IQR). The whiskers extending from each box indicate plus/minus 1.5 IQRs, and the points beyond the whiskers indicate outliers beyond 1.5 IQRs.

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  Figure 3. On‐aspirin agonist‐induced ex vivo platelet aggregation measurements were obtained in platelet‐rich plasma on day 7 of each treatment period 2 h following administration of the final aspirin dose. Aspirin doses of 81 mg, 162 mg, and 324 mg were administered for 7 days. Platelet aggregation is expressed as the percentage of maximal aggregation using platelet‐poor plasma as a referent. Adenosine diphosphate (ADP; 2 μM), collagen (2 μg/mL), epinephrine (10 μM), and arachidonic acid (AA; 1.6 mM) were used to stimulate platelet aggregation. The horizontal line within each box indicates the median; the top and bottom borders of each box indicate the interquartile range (IQR). The whiskers extending from each box indicate plus/minus 1.5 IQRs and the points beyond the whiskers indicate outliers beyond 1.5 IQRs.