A simple and reliable method is described for making permanent mounts of phytoplankton for critical microscopy and cell counting. Phytoplankton are fixed with glutaraldehyde, concentrated by gentle vacuum onto cellulosic membrane filters, and mounted directly in a water‐soluble, methacrylic resin. The resulting mounts are permanent and suitable for even very delicate nanoflagellates. Chlorophyll autofluorescence is preserved as is the structural integrity of cells. Resolution is very good and mounts can be examined with bright‐field, phase contrast, differential interference contrast, and fluorescence microscopy.
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William G. Crumpton (1987) studied this question.
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