Early developmental stage (7 days old) in mice was associated with substantial CV-B3 infection susceptibility and pathological severity compared to older mice, driven by CAR1/CAR2 expression.
Age-dependent decline in CAR1/CAR2 splice variants and NLRP3 inflammasome dysregulation explain the heightened susceptibility to severe CV-B3-induced myocarditis and encephalitis in early life.
Coxsackievirus B3 (CV-B3) infection causes inflammatory conditions such as viral myocarditis and meningitis, and incidence rates are rising annually. While children are more likely to be affected by severe manifestations, the molecular basis of this age-dependent susceptibility is poorly understood. In this study, we used young Balb/c mice at three developmental stages (7-, 14-, and 30-day-old mice) to investigate CV-B3 pathogenesis. Our findings revealed that 7-day-old mice exhibited substantial infection susceptibility and pathological severity compared to older mice. Critically, an age-dependent analysis showed a progressive decline in the expression of CV-B3-binding Coxsackievirus and Adenovirus Receptor (CAR) splice variants (CAR1 and CAR2) at both the transcriptional and translational levels as the mice matured from 7 to 30 days. These receptor isoforms demonstrated a direct correlation with viral replication efficiency in younger hosts. Concurrently, aging was associated with a rise in non-binding CAR variants (CAR3 and CAR4). During CV-B3 infection, the abundance of CAR1/CAR2 in young mice facilitated accelerated viral proliferation, coupled with the hyperactivation of the NLRP3 inflammasome and the expansion of IL-17-producing γδT cells (γδT17 cells). This cascade triggered excessive production of proinflammatory cytokines (IL-1β, IL-18, and IL-17), culminating in pronounced inflammatory infiltrates within cardiac and cerebral tissues. These findings establish NLRP3 inflammasome dysregulation as a critical determinant of CV-B3-induced tissue damage and provide novel insights into the heightened susceptibility to CV-B infection during early life and its associated severe disease rates.
Zhang et al. (Thu,) conducted a other in Coxsackievirus B3 (CV-B3) infection. Early developmental stage (7 days old) vs. Older developmental stages (14 and 30 days old) was evaluated on Infection susceptibility and pathological severity. Early developmental stage (7 days old) in mice was associated with substantial CV-B3 infection susceptibility and pathological severity compared to older mice, driven by CAR1/CAR2 expression.