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Companion note develops spectral optimisation methods and analysis of detector patterns for bioluminescence, suggesting new measurement approaches.
This companion progress note develops the original Firefly Interferometer proposal in two connected directions: spectral optimisation of the bioluminescent source and quantitative analysis of the external detector pattern. The delivered spectral photon distribution - not perceived colour - is treated as an adjustable experimental variable. The proposed development sequence begins with calibrated artificial sources, proceeds to controlled cell-free beetle luciferase systems, and considers a living bioluminescent source only where justified by the preceding results. No wavelength is assumed in advance to be superior. The detector wall is treated as a complete two-dimensional statistical distribution rather than an image judged by eye. Event-level impact positions and arrival times are evaluated through a frozen and blinded analysis pipeline. Fringe visibility, phase, spacing, envelope width, centroid, asymmetry, transmission and structured residuals are retained as interpretable observables. The methodology includes apparatus-stability controls, Poisson and overdispersed count models, delivered-spectrum calibration, pulse-matched artificial controls and a defined minimum detectable concealed difference. The objective is to determine whether a concealed spectral condition inside the sealed apparatus can be inferred solely from a reproducible change in the accumulated matter-wave detection pattern. No biological or matter-wave interferometry experiment is reported, and no empirical result is claimed. This publication is a separately dated companion to “The Firefly Interferometer: A Calibrated Test of Whether Bioluminescent Light Can Reduce Matter-Wave Interference in a Sealed System,” Version 1.0, DOI: 10.5281/zenodo.21417578. It develops, qualifies and operationally sharpens the original programme without altering its published file, publication date, DOI or historical record.
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