Freshly isolated tobacco mesophyll protoplasts had contents of K$, Cl− and Na$ slightly higher (on a cell basis) than the original leaf tissue, and had a high K$/Na$ ratio similar to that of the leaf tissue. Influxes of these ions into the protoplasts were of similar magnitudes to the corresponding fluxes in leaf tissue when compared on the basis of the respective plasmalemma surface areas. In both systems the K$ influx was strongly inhibited by CN− and by DNP. These results suggest that the ion relations of the freshly isolated mesophyll protoplasts were similar to those of the original leaf tissue and that isolated mesophyll protoplasts should be a useful system for the study of ion transport processes in leaf cells.
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Morris et al. (1980) studied this question.