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January 1, 2006Optics Express279 citationsOpen Access

Quantitative imaging of complex samples by spiral phase contrast microscopy

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SBStefan BernetAJAlexander JesacherSFSeverin Fürhapter

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Abstract

Recently a spatial spiral phase filter in a Fourier plane of a microscopic imaging setup has been demonstrated to produce edge enhancement and relief-like shadow formation of amplitude and phase samples. Here we demonstrate that a sequence of at least 3 spatially filtered images, which are recorded with different rotational orientations of the spiral phase plate, can be used to obtain a quantitative reconstruction of both, amplitude and phase information of a complex microscopic sample, i.e. an object consisting of mixed absorptive and refractive components. The method is demonstrated using a calibrated phase sample, and an epithelial cheek cell.

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Bernet et al. (2006) studied this question.

synapsesocial.com/papers/6a623842ce9bb37a6d7db2c9https://doi.org/10.1364/oe.14.003792
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