The potential of carbohydrate supramolecular hydrogels for long-term 3D culture of primary fibroblasts

-Nonyl-D-galactonamide fibers, which have the advantage of not being autofluorescent, do not mask the fluorescence of cells. But interestingly, they give a strong second harmonic generation (SHG) signal, due to their well-organized lamellar structure. We also made a special effort to visualize the penetration of cells within the depth of the hydrogels, in 3D, notably by sectioning the hydrogels, despite their softness. It was found that most of the cells stayed at the surface, but several cells grew within the supramolecular fiber network between 50 and 100 μm depth.