In Vitro Propagation of Lingonberry

Shoot proliferation of lingonberry ( Vaccinium vitis-idaea ssp. minus ) was achieved in vitro from shoot tips excised from 1-year-old stock plants and cultured on a low-salt medium supplemented with 2iP. Rooting was obtained without auxin application by placing microshoots in a peat-vermiculite medium in a high humidity chamber. After 6 weeks, the rooted plantlets were transplanted into a peat-lite mix, subjected to a 2-week low humidity acclimation process and subsequently acclimated in a greenhouse under 50% shade. Within 9 months, these plantlets developed a rhizomatous growth habit, in contrast to the nonrhizomatous habit of conventionally rooted cuttings. Chemical names used: N-(3-methyl-2-butenyl)-1 H -purin-6-amine (2iP).