Multiple shoot formation from shoot tips taken from juvenile rabbiteye blueberry ( Vaccinium ashei Reade) seedlings was induced on Knops medium with agar, casein hydrolysate, and N 6 -(∆ 2 -isopenteny1)-adenine (2iP) at 15 mg/liter. These shoots could be easily subcultured and rooted readily when cut off and inserted into a peat-perlite mixture under mist in the greenhouse. Nonjuvenile shoot tips from rabbiteye cultivars were difficult to establish in cultures. Successful establishment of some clones occurred by formation of fine, filamentous shoots which were as amenable to subculture as juvenile shoots. A 50-fold multiplication rate every 4 months was obtained with some clones.
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P. M. Lyrene (1980) studied this question.
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