What question did this study set out to answer?

This research examines how changes to the carbohydrate structures of rt-PA affect its clearance rate in the bloodstream.

The Influence of Carbohydrate Structure on the Clearance of Recombinant Tissue-Type Plasminogen Activator

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Randomized trial shows that carbohydrate modifications influence the clearance of rt-PA in rabbits, suggesting structural importance.

This research examines how changes to the carbohydrate structures of rt-PA affect its clearance rate in the bloodstream.
Applied sodium periodate to oxidize carbohydrate residues on rt-PA and assessed clearance rates in rabbits.
Used endo-beta-N-acetyl-glucosaminidase H to remove specific oligosaccharides from rt-PA and measured changes in clearance.
Created a mutant rt-PA with an amino acid substitution to disrupt glycosylation and evaluated its clearance.
Clearance rates decreased from 9.6 to 3.5 ml min-1 kg-1 after sodium periodate treatment (p<0.05).
Endo-H treatment reduced clearance to 5.0 ml min-1 kg-1, indicating sensitive dependence on oligosaccharide structures.
Production of rt-PA in CHO 15B cells increased clearance by 1.8-fold compared to conventional methods.

Hotchkiss et al. (1988) studied this question.

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