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January 1, 2015Journal of GenomicsOpen Access

Evaluation of the Performances of Ribosomal Database Project (RDP) Classifier for Taxonomic Assignment of 16S rRNA Metabarcoding Sequences Generated from Illumina-Solexa NGS

Giovanni Bacci Alessia Bani Marco Bazzicalupo Maria Teresa Ceccherini Marco Galardini P. Nannipieri Giacomo Pietramellara Alessio Mengoni

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Abstract

Here we report a benchmark of the effect of bootstrap cut-off values of the RDP Classifier tool in terms of data retention along the different taxonomic ranks by using Illumina reads. Results provide guidelines for planning sequencing depths and selection of bootstrap cut-off in taxonomic assignments.

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Bacci et al. (2015) studied this question.

synapsesocial.com/papers/6a62c79deed25793eac3ebcd https://doi.org/10.7150/jgen.9204

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