Immunologic Response of the Prostate to Bacteriuria and Bacterial Prostatitis II. Antigen Specific Immunoglobulin in Prostatic Fluid

No AccessJournal of Urology1 Jul 1982Immunologic Response of the Prostate to Bacteriuria and Bacterial Prostatitis II. Antigen Specific Immunoglobulin in Prostatic Fluid Jackson E. Fowler, and Michele Mariano Jackson E. FowlerJackson E. Fowler More articles by this author , and Michele MarianoMichele Mariano More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)52811-6AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail We used an indirect solid-phase radioimmunoassay to quantitate immunoglobulin binding to formalin-fixed Escherichia coli in expressed prostatic secretion (EPS). EPS samples from 20 men with no histories of bacteriuria, from eight men with histories of culture proven E. coli bacteriuria but with no clinical or bacteriologic evidence of prostatic infection, and from 5 men with culture proven E. coli bacterial prostatitis were assayed. Measurable binding of IgA to E. coli was observed for EPS samples from all the infected patients but from only 1 of the uninfected controls. Measurable binding of IgG to E. coli was observed for samples from 7 of 8 infected patients and from 2 of 10 uninfected controls. In no cases was there measurable IgM binding to E. coli. There was no measurable binding of IgA to formalin-fixed S. epidermidis; and no measurable binding of IgA to formalin-fixed K. pneumoniae, P. mirabilis or P. aeruginosa for samples from patients who had not been previously infected with these gram negative bacilli. Absorption of immune EPS with suspensions of these bacteria did not alter the binding of IgA to E. coli but absorption with a suspension of E. coli completely eliminated this binding. The magnitude and specificity of EPS immunoglobulin binding to E. coli was similar for the bacteriuric and bacterial prostatitis patients. Serum from 8 infected patients was assayed for immunoglobulin binding to E. coli. The per cent of serum IgG binding to E. coli for these patients was similar to that of EPS IgG, but the per cent of EPS IgA binding to E. coli was 3.5 to 147.5 times greater than that of serum IgA. In 1 patient with bacteriuria due to bacterial prostatitis, the concentrations of EPS IgG and EPS IgA binding to E. coli were 53 and 217 times greater, respectively, than those of a 1st voided urine specimen. Absorption of immune EPS with rabbit anti-human-secretory component indicated that>70 per cent of the IgA directed against E. coli was in the form of secretory-IgA. These data indicate that E. coli bacteriuria and E. coli bacterial prostatitis are associated with the local secretion of antigen specific IgA in prostatic fluid that appears to be independent of the systemic immune response. Further, these observations raise the possibility that the prostate gland is usually colonized during episodes of urinary tract infection but that symptomatic or chronic infection is prevented by the secretion of antigen specific IgA. © 1982 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByPfau A (2018) Re: Coagulase-Negative Staphylococcus in Chronic Prostatitis, by J. C. Nickel and J. W. Costerton, J. Urol., 147: 398–401, 1992Journal of Urology, VOL. 148, NO. 6, (1921-1922), Online publication date: 1-Dec-1992.Nickel J and Costerton J (2018) Coagulase-Negative Staphylococcus in Chronic ProstatitisJournal of Urology, VOL. 147, NO. 2, (398-400), Online publication date: 1-Feb-1992.Dairiki Shortliffe L, Elliott K and Sellers R (2018) Measurement of Urinary Antibodies to Crude Bacterial Antigen in Patients with Chronic Bacterial ProstatitisJournal of Urology, VOL. 141, NO. 3 Part 1, (632-636), Online publication date: 1-Mar-1989.Fowler J and Mariano M (2018) Longitudinal Studies of Prostatic Fluid, Immunoglobulin in Men With Bacterial ProstatitisJournal of Urology, VOL. 131, NO. 2, (363-369), Online publication date: 1-Feb-1984.Fowler J and Mariano M (2018) Bacterial Infection and Male Infertility: Absence of Immunoglobulin A with Specificity for Common Escherichia Coli 0-Serotypes in Seminal Fluid of Infertile MenJournal of Urology, VOL. 130, NO. 1, (171-174), Online publication date: 1-Jul-1983. Volume 128Issue 1July 1982Page: 165-170 Advertisement Copyright & Permissions© 1982 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Jackson E. Fowler More articles by this author Michele Mariano More articles by this author Expand All Advertisement Loading ...