The detection of 13C is now amenable to high-resolution NMR spectroscopy of proteins. A protocol for 13C homonuclear decoupling in multidimensional experiments correlates carbonyl, Cα, and Cβ nuclei (see picture). These techniques also allow the detection of side-chain nuclei in exclusively heteronuclear experiments, and thus the complete assignment of proteins.
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Bermel et al. (2005) studied this question.
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