Dual‐Functional Engineered Lactobacillus Rhamnosus for Blocking the Dissemination of Drug Resistance

Drug‐resistant pathogens critically endanger lives and threaten global public health. While traditional microbicidal materials can eliminate pathogens, residual drug‐resistant genes (DRGs) persist in the environment, potentially spreading resistance via horizontal gene transfer. Consequently, developing new microbiocidal strategies with bacteria and DRGs elimination abilities becomes an urgent priority. Here, we fabricate an integrated living material (LG@PCeO 2 ) by engineering Lactobacillus rhamnosus (LG) with polydopamine (PDA) and in situ grown CeO 2 nanoparticles. This bioactive material simultaneously eradicates drug‐resistant pathogens and cleaves DRGs, effectively blocking the transmission of resistance. The LG@PCeO 2 killed methicillin‐resistant Staphylococcus aureus (MRSA) and inhibited/removed corresponding biofilms effectively by synergizing the inherent metabolic properties of LG and the CeO 2 ‐enhanced electrostatic effects. Moreover, the PDA coating endows antioxidant capability, while the in situ‐grown CeO 2 confers DNase‐mimicking activity, enabling degradation of extracellular and genomic DRGs for LG@PCeO 2 . In an MRSA‐infected wound model, LG@PCeO 2 not only concurrently killed bacteria and eliminated residual DRGs within infected tissue but also inhibited inflammation to effectively block the spread of drug resistance and accelerate wound healing synchronously. This bioactive material offers a promising living‐material strategy to combat drug resistance.