Population
Bacterial sodium channel NaChBac
Comparison
Paired cysteine substitutions for the third… vs Single cysteine mutants
Design
Preclinical
Key result
Paired cysteine substitutions (D60C:R3C) in the bacterial sodium channel NaChBac form a disulfide bond during activation, revealing voltage-dependent formation of an ion pair in real time.
Authors
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Supports bacterial NaV gating model; leaves open relevance to human cardiac channelopathies.
Demonstrates real-time voltage-dependent formation of an ion pair during activation of the voltage sensor, suggesting this interaction catalyzes S4 movement.
DeCaen et al. (2008) studied this question. Paired cysteine substitutions (D60C:R3C) in NaChBac vs. Single cysteine mutants was evaluated on Disulfide bond formation and channel activation kinetics. Paired cysteine substitutions (D60C:R3C) in the bacterial sodium channel NaChBac form a disulfide bond during activation, revealing voltage-dependent formation of an ion pair in real time.