Micropropagation of Spinacia oleracea L. through Culture of Shoot Primordia.

Tissue-cultured shoot primordia of Spinacia oleracea were induced from apical domes of shoot tips in MS liquid medium supplemented with 0.2, 2.0 or 4.0mg/l IAA and 0.2, 2.0 or 4.0mg/l BAP at pH 5.8 by shaking at 2 cycles/minute on rotary culture equipment (1m diameter). Multiplication of those shoot primordia was the most effective in MS liquid medium supplemented with 0.2mg/l IAA and 0.02mg/l BAP. Vigorous regeneration of plantlets was accelerated in MS supplemented with 0.2mg/l BAP and 4.0mg/l IAA; 2.0mg/l BAP and 0.02, 0.2, 1.0 or 4.0mg/l IAA; or 4.0mg/l BAP and 0, 0.02, or 0.2mg/l IAA in combination, and their root formation was easily promoted in MS supplemented with no hormone or 0.2mg/l IAA.