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A solvent system was developed for the isolation of 3 major Alternaria mycotoxins by high performance liquid chromatography (HPLC). Alternariol (AOH), alternariol methyl ether (AME), and altenuene (ALT) were purified using either a semipreparative or a preparative HPLC column. Gram quantities of pure mycotoxins were obtained in a single preparative HPLC step. Distribution of other individual mycotoxins and metabolites in various fractions obtained from either chromatographic procedure is discussed.
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Chu et al. (1981) studied this question.
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