Transcription Factor E2 Regulates BPV-1 DNA Replication In Vitro by Direct Protein-Protein Interaction

In eukaryotes, DNA replication is limited to a certain time in the cell cycle called S phase. The initiation of DNA replication is also regulated in such a way as to allow one and only one initiation event in each region of the cellular DNA (Kriegstein and Hogness 1974). Elegant in vivo assays that can distinguish between one or two copies of a plasmid per yeast cell have shown that overreplication of such an individual ARS-containing plasmid occurs at a frequency of less than 1 in 1000 mitotic divisions (Koshland et al. 1985). Such tight quantitative and temporal regulation is likely to be mediated by different and multiple pathways, whereby each level of regulation affords a certain amount of protection against a mistake. Thus, the regulation of the initiation of DNA synthesis in eukaryotes is likely to be as complicated as is the control of gene expression, and many aspects...