Determination of Organic Acids in Dairy Products by High Performance Liquid Chromatography

A high performance liquid chromatographic method was developed for the quantitative analysis of organic acids in dairy products. A reverse‐phase C8 column at room temperature, a mobile phase of 0.5% w/v buffer ((NH 4 ) 2 HPO 4 at pH 2.24 with H 3 , PO 4 )‐0.4% v/v acetonitrile, UV detection at 214 nm and 1.2 mL/min flow rate were utilized. Formic, acetic, pyruvic, propionic, uric, orotic, citric, lactic and butyric acids were quantitated for raw milk, yogurt, Blue, Provolone, Port Salut and Quartirolo cheeses. Recoveries greater than 85.3% were observed for all acids.