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Double-chain derivatives of orotic acid (1) and hydroorotic acid (2) formed stable monolayers at the air-water interface. FT-IR and ESCA spectroscopies revealed specific cooperative binding of adenine from the aqueous subphase to monolayer 1 in preference to thymine. Monolayer 2 did not bind these nucleic acid bases.
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Kawahara et al. (1992) studied this question.
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