A rapid increase in the concentration of intracellular free calcium ([Ca++i]) in B cells after mIg crosslinking has been documented previously by fluorimetric analysis of cell populations loaded with the fluorescent Ca++ indicator Quin 2. Although providing a valuable indication of Ca++ mobilization in the population as a whole, it has not been possible to determine whether only a subpopulation of the cells or the entire population exhibits this response. In this report, we describe the marriage of flow cytometry and Quin 2 technology, which permits discrimination of Ca++ mobilization by subpopulations of cells, as well as in whole populations. We have determined that the entire mIg+ population exhibits a synchronous increase in [Ca++i] rapidly after stimulation. Furthermore, all members of the population appear to undergo an approximately equal response.
No takes yet. Share an insight, caveat, or question.
Ransom et al. (1986) studied this question.