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September 11, 2014Cardiovascular ResearchOpen Access

Emergence of Orai3 activity during cardiac hypertrophy

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Population

Normal and hypertrophied adult rat ventricular cardiomyocytes

Comparison

In vivo delivery of Cy3-tagged siRNAs for… vs Control isolated cardiomyocytes

Design

Preclinical

Key result

Specific knockdown established Orai3, but not Orai1, as the critical partner of STIM1 carrying voltage-independent Ca(2+) entries in adult hypertrophied rat cardiomyocytes.

Authors

YSYouakim SalibaMKMathilde KeckAMAlexandre Marchand

Discussion

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Overview

No immediate clinical implications; leaves open Orai3 as a research target in hypertrophy models.

Structured PICO

P
Population
Normal and hypertrophied adult rat ventricular cardiomyocytes
I
Intervention
In vivo delivery of Cy3-tagged siRNAs for specific Orai1 and Orai3 knockdown
C
Comparator
Control isolated cardiomyocytes
O
Outcome
Voltage-independent (store-independent and store-operated) Ca(2+) entries and Orai-mediated currentssurrogate

Orai3 is identified as the essential partner of STIM1 driving voltage-independent calcium entry and arachidonic acid-activated currents in adult cardiomyocytes during hypertrophy.

Cite This Study

Saliba et al. (2014) studied Cardiac hypertrophy. Orai1 and Orai3 knockdown via siRNA vs. Control isolated cardiomyocytes was evaluated on Voltage-independent Ca(2+) entries and Orai-mediated currents. Specific knockdown established Orai3, but not Orai1, as the critical partner of STIM1 carrying voltage-independent Ca(2+) entries in adult hypertrophied rat cardiomyocytes.

synapsesocial.com/papers/6a652dd6d4d8171944cb2de6https://doi.org/10.1093/cvr/cvu207
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