Key result
Knockdown of Orai1 or STIM1 using shRNA inhibited cell proliferation and markedly reduced neointima formation 14 days post balloon injury in rat carotid arteries.
Why the study?
Does Orai1 knockdown prevent neointima formation and VSMC proliferation following vascular injury in rodent models?
Population
Rat carotid arteries subjected to balloon injury, mouse carotid arteries subjected to ligation, and vascular…
Comparison
Lentiviral particles encoding short-hairpin RNA… vs Control shRNA targeted to luciferase
Design
Preclinical
Follow-up
14 days
Authors
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Orai1 knockdown may attenuate post-injury remodeling in rodents; leaves open therapeutic translation to human restenosis.
Does Orai1 knockdown prevent neointima formation and VSMC proliferation following vascular injury in rodent models?
Orai1 is upregulated during vascular injury and its knockdown prevents neointima formation and VSMC proliferation, identifying it as a potential therapeutic target for vascular remodeling.
Zhang et al. (2011) studied Vascular injury and neointima formation. shRNA targeting Orai1 (shOrai1) or STIM1 (shSTIM1) vs. Control shRNA targeted to luciferase (shLuciferase) was evaluated on Neointima formation and cell proliferation. Knockdown of Orai1 or STIM1 using shRNA inhibited cell proliferation and markedly reduced neointima formation 14 days post balloon injury in rat carotid arteries.
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