Pyruvate (10 mM) increased the recovery of left ventricular developed pressure from 57.2% to 98.9% (P<0.05) and reduced mitochondrial 2-DG entrapment in ischemic rat hearts.
Does pyruvate reduce mitochondrial permeability transition and improve functional recovery in isolated rat hearts subjected to ischemia and reperfusion?
Pyruvate enhances functional recovery and reduces mitochondrial permeability transition in isolated rat hearts subjected to ischemia-reperfusion injury.
Absolute Event Rate: 98.9% vs 57.2%
p-value: p=<0.05
We have used mitochondrial entrapment of 2-deoxy-D-3Hglucose (2-DG) to demonstrate that recovery of Langendorff-perfused rat hearts from ischemia is accompanied by reversal of the mitochondrial permeability transition (MPT). In hearts loaded with 2-DG before 40 min of ischemia and 25 min of reperfusion, 2-DG entrapment expressed as 10(5) x (mitochondrial 2-[3HDG dpm per unit citrate synthase)/(total heart 2-3HDG dpm/g wet wt)] increased from 11.1 +/- 1.3 (no ischemia, n = 4) to 32.5 +/- 1.9 (n = 6; P < 0.001). In other experiments, 2-DG was loaded after 25 min of reperfusion to determine whether some mitochondria that had undergone the MPT during the initial phase of reperfusion subsequently "resealed" and thus no longer took up 2-DG. The reduction of 2-DG entrapment to 20. 6 +/- 2.4 units (n = 5) confirmed that this was the case. Pyruvate (10 mM) in the perfusion medium increased recovery of left ventricular developed pressure from 57.2 +/- 10.3 to 98.9 +/- 10.8% (n = 6; P < 0.05) and reduced entrapment of 2-DG loaded preischemically and postischemically to 23.5 +/- 1.5 (n = 4; P < 0. 001) and 10.5 +/- 0.5 (n = 4; P < 0.01) units, respectively. The presence of pyruvate increased tissue lactate content at the end of ischemia and decreased the effluent pH during the initial phase of reperfusion concomitant with an increase in lactate output. We suggest that pyruvate may inhibit the MPT by decreasing pHi and scavenging free radicals, thus protecting hearts from reperfusion injury.
Kerr et al. (Mon,) conducted a other in Ischemia/reperfusion injury. Pyruvate vs. Control medium was evaluated on Recovery of left ventricular developed pressure (%) (p=<0.05). Pyruvate (10 mM) increased the recovery of left ventricular developed pressure from 57.2% to 98.9% (P<0.05) and reduced mitochondrial 2-DG entrapment in ischemic rat hearts.