Genome‐Wide Screening of Phase Separation Proteins and Functional Dissection of Candidate Proteins for Saline‐Alkali Stress Tolerance in Soybean

Summary statement We designed a bioinformatics pipeline combining PSPredictor and Metapredict to systematically identify phase separation proteins in soybean, yielding a total of 5515 candidates. Experimental validation confirmed that four nuclear‐localized proteins are capable of forming dynamic liquid‐like condensates through liquid–liquid phase separation both in vivo and in vitro. In addition, saline‐alkali tolerance assays revealed that GmBAF60b, GmFCA and GmPUX5 significantly enhance stress tolerance by lowering Na + /K + ratios and boosting antioxidant enzyme activities. These findings provide key genetic resources and mechanistic insights into LLPS‐mediated stress adaptation in crops.