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Abstract Zinc deficiency (ZnD) is a major risk factor for metabolic and inflammatory diseases associated with gut microbial alterations, such as obesity, type 2 diabetes, and inflammatory bowel disease. Despite its importance, there are no established dietary recommendations for zinc supplementation in individuals with these conditions, except in cases of severe diarrhea in children. This gap stems from inconsistent outcomes in zinc supplementation trials, suggesting an incomplete understanding of zinc-mediated spatial and temporal regulation within the host-microbiome interface. This study employed a multiomics approach to investigate zinc-driven host-gut microbial interactions in the intestinal tract of mice fed zinc-adequate or ZnD diets. Radio tracing and metallomics analyses uncovered differential zinc abundance across intestinal tissues, with conventionalized germ-free mice displaying significantly lower zinc levels than germ-free mice, highlighting the reciprocal regulation of zinc between host tissues and gut microbes. Transcriptomic analyses revealed region-specific effects of ZnD, including altered energy metabolism and apoptosis in the small intestine, and impaired barrier function and redox processes in the colon. Metagenomics profiling revealed that ZnD reduced microbial diversity in the small intestine, while the cecum and colon were protected from diversity loss but exhibited an increased abundance of pathogenic bacteria. Correlation analyses linked tissue-specific host gene expression to shifts in microbial populations, identifying potential microbial mediators of host transcription changes under ZnD. Collectively, these findings emphasize the critical role of zinc in spatially regulating host-microbiome interactions, advancing our understanding of region-specific impacts of ZnD on the GI tract and disease risk. Abstract Figure
Sastry et al. (Wed,) studied this question.