Randomized trial evaluates stanozolol's effects on myogenic differentiation in porcine muscle cells, suggesting its potential in cultured meat production.
Key Points
This study evaluates the effects of stanozolol on myogenic differentiation and myotube formation in porcine muscle satellite cells.
PMSCs were treated with stanozolol at concentrations of 0.1, 0.5, and 1 μM.
Cell viability and myogenic differentiation markers were assessed after treatment.
Signaling pathways related to hypertrophy and atrophy were analyzed.
Stanozolol did not affect cell viability or proliferation in the tested concentrations.
It significantly increased the expression of myogenic markers MyoD, MyoG, and MyHC in a concentration-dependent manner.
At 1 μM, stanozolol promoted larger multinucleated myotube formation and modulated key signaling pathways.