Transcriptomic characterization of mesenchymal and skin tissues in Cervus elaphusxanthopygus antler and identification of growth-related candidate genes
The central aim is to systematically compare transcriptomic profiles of skin and mesenchymal tissues to identify candidate genes that regulate antler growth.
Performed transcriptome sequencing on antler tissues using Illumina NovaSeq 6000.
Conducted bioinformatics analysis to identify gene expression levels.
Utilized fluorescence quantitative PCR to validate the expression of select genes.
Generated 109.82 Gb of transcriptome data with 40,023 transcripts from deer antlers.
Identified 3,530 genes with differential expression between skin and mesenchymal tissues.
Highlighted key genes involved in rapid growth, including MDM2, RPS6KB1, BMP4, and others.