Degummed silk was dissolved in a mixture of CaCl2 and ethanol at low temperature. The concentration and pH of the centrifuged, filtered, and dialyzed silk solution were adjusted, and then it was kept in an air-conditioned room for gelation. The mechanism of the gelation and the gel structure were studied. It was found that the gel was formed due to the formation of beta-structure in the fibroin solution. The gelation was dependent on the pH of the fibroin solution.
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Ayub et al. (1993) studied this question.
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