Dysregulation in the Expression of Platelet Surface Receptors in Acute Coronary Syndrome Patients—Emphasis on P2Y12

Figures

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  Figure 1. The mRNA expression levels of P2Y12, GP1BB, ITGA2B, ITGB3, and F2R in platelets of ACS patients compared with the control group. The relative expression of the selected genes was illustrated with −ΔCt value. 18S rRNA was used as a reference gene. Data are plotted as individual values with horizontal bars presenting the mean. The p-values reported above the graphs represent the results of the Student’s t-test, or, in cases of unequal variance, Welch’s t-test. F2R was not statistically significant with p = 0.074.

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  Figure 2. Levels of P2Y12, GPIIIa, and GPIb proteins in blood platelet lysates from ACS patients compared with the control group. Data are plotted as individual values with horizontal bars presenting the mean concentration. The p-values reported above the graphs represent the results of t-tests following log-transformation.

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  Figure 3. Platelet surface P2Y12 expression measured by flow cytometry method using PE Anti-Human P2Y12. Results presented in the figure show the percentage of P2Y12-positive targets in the population of CD61-positive subjects. Data are presented as a column bar graph with mean ± SD.

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  Figure 4. Confocal microscopy of fixed platelets. Samples were labeled with the platelet marker CD61-PerCP-Cy5.5 and with P2Y12-PE. Control and study platelets were deposited on the glass slides with CyGEL™. (A) blood platelets obtained from control donor; (B) blood platelets obtained from ACS patient.

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  Figure 5. The relative expression of miR-223-3p and miR-126-3p in blood platelets of the study and control group. Data were calculated using a −ΔCt value with the means of both miR-191-3p and cel-miR-39-3p as reference miRNAs. Data are plotted as individual values with horizontal bars presenting the mean. The p-values reported above the graphs represent the results of Student’s t-tests or, in cases of unequal variance, Welch’s t-test.