What should be considered to get a lot of adipose derived stem cells?

Adipose derived stem cells (ASCs) are derived from mesenchyme and have several general or unique characteristics. ASCs have more colony frequency, higher proliferation capacity and can be cultured longer compared with bone marrow-derived mesenchymal stem cells. The yield, frequency of ASCs, the number of viable cells, proliferation and differentiation potential, and growth characteristics of ASCs have various spectrum according to the methods of harvesting, adipose tissue site for harvesting, and patient's individual characteristics.