Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.
Key result
Compensatory mutations in human U2 snRNA suppressed branch site mutations in SV40 early pre-mRNA, establishing that recognition of the mammalian pre-mRNA branch site by U2 snRNP involves base-pairing.
Population
SV40 early pre-mRNA and cloned human U2 gene (molecular model)
Comparison
Mutations in the small t pre-mRNA branch site… vs Wild-type/unmutated sequences
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Supports U2-branch site pairing in splicing; leaves open its role in cardiac alternative splicing regulation.
Structured PICO
P
Population
SV40 early pre-mRNA and cloned human U2 gene (molecular model)
I
Intervention
Mutations in the small t pre-mRNA branch site (UUCUAAU) and compensatory mutations in a cloned human U2 gene (nucleotides 34-36)
C
Comparator
Wild-type/unmutated sequences
O
Outcome
Small t splicing relative to large T splicingsurrogate
This study establishes that recognition of the poorly conserved mammalian pre-mRNA branch site sequence by U2 snRNP involves RNA-RNA base-pairing.
Cite This Study
Wu et al. (1989) studied this question. U2 snRNA compensatory mutations vs. Wild-type U2 snRNA / unmutated branch site was evaluated on Small t splicing relative to large T. Compensatory mutations in human U2 snRNA suppressed branch site mutations in SV40 early pre-mRNA, establishing that recognition of the mammalian pre-mRNA branch site by U2 snRNP involves base-pairing.