Randomized trial investigates how TCR influences CD4+ TRM generation in lungs after influenza, indicating potential therapeutic insights.
Key Points
This research aims to understand how the T-cell receptor (TCR) contributes to the formation of lung CD4+ tissue-resident memory T cells after influenza infection.
Utilized a TCR transgenic mouse line (GPB1) expressing a fixed TCRB chain after influenza infection
Transferred GPB1 T cells into WT hosts followed by challenges with recombinant PR8 expressing GP61-80
Monitored TCR signal strength using a Nur77-GFP reporter.
Lung CD4+ T cells showed higher TCR avidity and signal strength compared to those in mediastinal lymph nodes.
TCR signal strength correlated with increased expression of integrin CD49b and chemokine receptor CXCR6 in lung TRM.
High avidity CD4+ TRM were established in the lung following primary and heterologous secondary challenges.