The L-2,3-butanediol dehydrogenase produced in E. coli JM109/pLBD2-CTC was purified by 5 steps. The molecular mass of this enzyme was estimated at 110 kDa and the subunit was measured to be 30 kDa. The L-BDH had some differences from the BDHs from other sources in substrate specificity, pI value, pH stability, effects of divalent cations, and organic acids.
No takes yet. Share an insight, caveat, or question.
Takusagawa et al. (2001) studied this question.
Synapse has enriched 5 related papers. Consider them for comparative context: