Angiotensin-(1-7) Central Mechanisms After ICV Infusion in Hypertensive Transgenic (mRen2)27 Rats

Figures

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  FIGURE 1. Representative photomicrographs of sections of the left ventricle of sections control (SD saline, n = 5); (A), control treated with Ang-(1-7) (SD A7, n = 4); (B), hypertensive rats control (TG saline, n = 4); (C) and hypertensive rats treated with ICV Ang-(1-7) (TG A7, n = 5); (D). Bar = 20 μm. Stain: hematoxylin/eosin. The cardiomyocyte diameter (E) of the free wall LV and Hydroxyproline as a fibrotic marker (F). *p < 0.05 vs. SD saline; #p < 0.05 vs. SD A7; †p < 0.05 vs. TG saline (One-way ANOVA followed by Newman-Keuls post hoc test).

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  FIGURE 2. Concentrations of pro-inflammatory cytokines in the hypothalamus in SD and hypertensive rats treated with Ang-(1-7) ICV. interleukin-1 alpha (IL-1α) (A), interleukin-6 (IL-6) (B), tumor necrosis factor-alpha (TNF-α) (C) and anti-inflammatory cytokine, interleukin-10 (IL-10) (D) were measured using commercially available antibodies by enzyme-linked immunosorbent assay (ELISA). Results are expressed as mean ± SEM. n = 5 or 6. *p < 0.05 vs. SD saline; # p < 0.05 vs. SD A7; †p < 0.05 vs. TG saline (One-way ANOVA followed by Newman-Keuls post hoc test).

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  FIGURE 3. Expression or activity of renin-angiotensin components in the hypothalamus in SD and hypertensive rats treated with Ang-(1-7) ICV. AT1 receptor (A), AT2 receptor (B), Mas receptor (C), ACE activity (D), and ACE2 activity (E). Results are expressed as mean ± SEM. n = 4 or 5. *p < 0.05 vs. SD saline; #p < 0.05 vs. SD A7; †p < 0.05 vs. TG saline (One-way ANOVA followed by Newman-Keuls post hoc test).

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  FIGURE 4. Expression of mRNA for iNOS in the hypothalamus in SD and hypertensive rats treated with Ang-(1-7) ICV. Results are expressed as mean ± SEM. n = 4 or 5. *p < 0.05 vs. SD saline; #p < 0.05 vs. SD A7; †p < 0.05 vs. TG saline (One-way ANOVA followed by Newman-Keuls post hoc test).

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  FIGURE 5. Representative 2-DE gel image of DIGE-stained proteins (gray view). The represented gel is from the TG CT group and the squares indicate the identified spots. The panel on the right shows the number of the matched spots identified in each analyzed group, where (-) means not detected. The panel also include the gene from the identified protein. Pdia3, protein disulfide-isomerase A3; Uchl1, ubiquitin carboxyl-terminal hydrolase isozyme L1; Prdx2, peroxiredoxin-2; Pebp1/HCNP, phosphatidylethanolamine-binding protein 1; Prdx1, peroxiredoxin-1; Ppia/CypA, peptidyl-prolyl cis-trans isomerase A/cyclophilin A.