Structural features of mossy cells in the hamster dentate gyrus, with special reference to somatic thorny excrescences

We have recently revealed that large multipolar neurons, presumed mossy cells in the hamster dentate gyrus (DG), were calretinin (CR)-immunoreactive (IR) at the ventral level, although these neurons were CR-negative at the dorsal level. In the present study, we confirmed this identification with several methods and analyzed structural features of hamster mossy cells in detail. Golgi impregnationi and intracellular Lucifer yellow labeling studies revealed that mossy cells in the hamster dentate hilus had extraordinarily prominent thorny excrescences on their somata as well as on their proximal dendrites. Mossy cells exhibited dorsoventral differences in their structural features; proximal dendrites of single mossy cells were fewer, and thorny excrescences were larger and more complicated at the dorsal level than at the ventral level. Electron microscopic serial section three-dimensional reconstructions revealed that somatic thorny excrescences consisted of large and complicated spines, which received numerous asymmetrical synapses from mossy fiber terminals. In addition, our confocal laser scanning microscopic observations also revealed many glutamic acid decarboxylase-immunoreactive punctae abutting the mossy cell somata and dendrites. Our present and previous observations revealed the structural features of hamster mossy cells and their differences along the dorsoventral axis and further indicated that mossy cells were prominently different in their chemical and morphological features among species.