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January 1, 1986Journal of Biological ChemistryOpen Access

At high concentrations of myosin light chain kinase, a second site on the 20,000-dalton light chain of smooth muscle myosin is phosphorylated, identified as threonine 18.

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Population

Turkey gizzard myosin (20,000-dalton light chain of smooth muscle myosin)

Design

Preclinical

Key result

At high concentrations of myosin light chain kinase, a second site on the 20,000-dalton light chain of smooth muscle myosin is phosphorylated, identified as threonine 18.

Authors

MIMitsuo IkebeDHDavid J. HartshorneMEMarshall Elzinga

Discussion

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Overview

Supports expanded MLCK substrate mapping in vitro; leaves open physiological or therapeutic relevance in vascular smooth muscle.

Structured PICO

P
Population
Turkey gizzard myosin (20,000-dalton light chain of smooth muscle myosin)
I
Intervention
Phosphorylation by myosin light chain kinase
O
Outcome
Identification of the second phosphorylation site and kinetics of phosphorylation and dephosphorylationsurrogate

Identifies threonine 18 as the second phosphorylation site on the 20,000-dalton light chain of smooth muscle myosin by myosin light chain kinase and details its kinetics.

Cite This Study

Ikebe et al. (1986) studied this question. Myosin light chain kinase was evaluated on Phosphorylation of a second site on the 20,000-dalton light chain of smooth muscle myosin. At high concentrations of myosin light chain kinase, a second site on the 20,000-dalton light chain of smooth muscle myosin is phosphorylated, identified as threonine 18.

synapsesocial.com/papers/6a6c094945e10bb5bcf311aehttps://doi.org/10.1016/s0021-9258(17)42425-2
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