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The aromatic compounds used in a variety of industrial products are currently obtained from nonrenewable petroleum sources. Alternatively, the plant polymer lignin is an abundant renewable source of aromatics, and its depolymerization generates a variety of products that can include acetovanillone, a vanillin derivative containing an acetyl side chain. The Alphaproteobacterium Novosphingobium aromaticivorans DSM12444 can metabolize several chemically modified aromatics in deconstructed lignin, but not acetovanillone. In this work, adaptive laboratory evolution identified a single amino acid change in the previously uncharacterized gene product Saro₁862 that is necessary and sufficient for N. aromaticivorans growth with acetovanillone as a sole growth substrate, as well as other aromatic monomers not metabolized by wildtype cells. We show that a glutamate (E) to lysine (K) substitution at amino acid residue 16 of Saro₁862 results in a ∼1600-fold increase in the rate of ATP-dependent acetovanillone phosphorylation. We also find that recombinant Saro₁862 E16K phosphorylates several other aromatic compounds in vitro, defining the first reported catalytic activity for the widespread UPF0261 protein domain contained in Saro₁862. Thus, we propose naming Saro₁862 MarK, for multiple aromatic kinase. A 1. 57 Å crystal structure of MarK E16K predicts that the E16K substitution lies in a potential ATP binding site, suggesting how this amino acid change increased catalytic activity. A search for homologs of MarK and other proteins required for acetovanillone degradation predicts that this pathway for aromatic metabolism exists throughout the bacterial phylogeny.
Hall et al. (Wed,) studied this question.