Characterization of the Complete Nucleotide Sequences of IMP-4-Encoding Plasmids, Belonging to Diverse Inc Families, Recovered from Enterobacteriaceae Isolates of Wildlife Origin

The complete nucleotide sequences of six IMP-4-encoding plasmids recovered from Enterobacteriaceae isolates of wildlife origin were characterized. Sequencing data showed that plasmids of different incompatibility groups (IncM, IncI1, IncF, and nontypeable [including an IncX5_2 and two pPrY2001-like]) carried the bla IMP-4 -carrying integrons In809 or In1460. Most of the plasmids carried an mph (A) region, and chrA -like, aac(3)-IId , and bla TEM-1b genes. Finally, plasmid analysis revealed the involvement of two different IS 26 - and Tn 1696 -associated mechanisms in the mobilization of IMP-4-encoding integrons.