Answers are grounded in this paper and cite their sources.
Reveals a novel method for real-time quantification of cellular uptake and release using luciferin-conjugates.
The design, synthesis, and evaluation of conjugates of arginine-rich transporters and luciferin are described that release luciferin only after entry into cells that are stably transfected with luciferase. Each molecule of free luciferin that is released after entry generates a photon that can be measured allowing for real-time quantification of uptake and release in cells. The process provides a method to assay uptake and release of free luciferin as a function of variations in the releasable linker and in the transporter.
No takes yet. Share an insight, caveat, or question.
Jones et al. (2006) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: