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January 5, 2005Journal of Biological ChemistryOpen Access

The Elasticity of Individual Titin PEVK Exons Measured by Single Molecule Atomic Force Microscopy

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Key result

Engineered PEVK proteins from exons 161, 120, and 184 exhibited similar persistence lengths (0.92, 0.89, and 0.98 nm), indicating similar elastic properties regardless of proline content.

Population

Engineered PEVK proteins (polypeptides encoded by individual titin PEVK exons 161, 120, and 184)

Comparison

Measurement of persistence length using single… vs Comparison among polypeptides with varying…

Design

Preclinical

Authors

ASAtom SarkarSCSofia CaamanoJFJulio M. Fernández

Discussion

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Overview

PEVK exon diversity shapes titin elasticity; leaves open therapeutic targeting of passive stiffness in cardiomyopathy.

Structured PICO

P
Population
Engineered PEVK proteins (polypeptides encoded by individual titin PEVK exons 161, 120, and 184)
I
Intervention
Measurement of persistence length using single molecule atomic force microscopy
C
Comparator
Comparison among polypeptides with varying proline contents (7%, 14%, and 37%) and total PEVK contents (55%, 70%, and 87%)
O
Outcome
Persistence length (p) of the engineered PEVK proteinssurrogate

Individual titin PEVK exons encode polypeptides with similar elastic properties regardless of proline content, indicating that alternative splicing solely adjusts the length of the PEVK domain.

Cite This Study

Sarkar et al. (2005) studied this question. Engineered PEVK proteins (exons 161, 120, and 184) was evaluated on Persistence length (p) of the engineered PEVK proteins. Engineered PEVK proteins from exons 161, 120, and 184 exhibited similar persistence lengths (0.92, 0.89, and 0.98 nm), indicating similar elastic properties regardless of proline content.

synapsesocial.com/papers/6a6cdbf0660549caf2c2a9fchttps://doi.org/10.1074/jbc.c400573200
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