Feasibility of quantitative determination of doxorubicin with surface‐enhanced Raman spectroscopy

Surface‐enhanced Raman spectroscopy (SERS) was performed using excitation at 488 nm in a blood plasma–doxorubicin–silver colloid system. With a blood plasma content of 1%, a partial least‐squares calibration of the doxorubicin was made in the 10–750 n M range. Predictions for a test set generated a root mean square error of prediction of 70 n M . The use of SERS and chemometrics in complex systems made it possible to use the highly informative Raman signals even at low concentrations without the need for sample pretreatment such as extraction.