Study on Slice-based Biosensor for Electrophysiological Propagation Measurement in Drugs Screening

In order to investigate the signal propagation of the cardiac tissue and the action of drugs, we developed a slice- based biosensor to monitor the electrophysiological activities of the cardiac tissues. At first, we analyzed the interface model of the slice-based biosensor and the electrolyte, and observed the characteristics of the slice-based biosensor with tissue over time. Then, using the slice-based biosensor we sampled the beating signals of the cardiac slices respectively in the presence of the normal Tyrode’s solution, Adrenaline Hydrochloride and Acetylcholine chloride. Further, we used statistic analysis and correlation analysis to review the propagation of beating signals and the action of drugs on propagation. Results showed that the slice-based biosensor is apt and stable enough to catch the character and tendency of signal propagation and Delay is the key to deduce the conduction tendency. Adrenaline Hydrochloride and Acetylcholine chloride respectively have excitatory and inhibiting action on Amplitude and Frequency of the beating signals, and appear dependent on dosage. Thus, slice-based biosensor offers a noninvasive and versatile method to study the cardiac beating and the electrophysiological propagation for drugs screening. It is significative to guide clinical remedy of the drugs’ acting targets in the future.