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January 1, 2013Journal of Diabetes ResearchOpen Access

Poorly controlled diabetes (~15 mM glucose) exacerbated GPVI-dependent platelet ROS generation and calcium flux, which was reversed to healthy levels by the Syk inhibitor BAY61-3606.

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Why the study?

Does glycaemic control or Syk inhibition reduce GPVI-dependent platelet hyperreactivity in a rhesus monkey model of Type 1 diabetes?

Population

Nonhuman primate (rhesus monkey) model of Type 1 diabetes.

Comparison

Poorly controlled diabetes and in vitro… vs Well-controlled diabetes and healthy monkeys.

Design

Preclinical

Key result

Poorly controlled diabetes (~15 mM glucose) exacerbated GPVI-dependent platelet ROS generation and calcium flux, which was reversed to healthy levels by the Syk inhibitor BAY61-3606.

Authors

JAJ F ArthurYSYuan ShenYCYe Chen

Discussion

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Member takes

Overview

May inform GPVI signaling in diabetic thrombosis; leaves open human translation and therapeutic implications.

Structured PICO

Does glycaemic control or Syk inhibition reduce GPVI-dependent platelet hyperreactivity in a rhesus monkey model of Type 1 diabetes?

P
Population
Nonhuman primate (rhesus monkey) model of Type 1 diabetes.
I
Intervention
Poorly controlled diabetes (blood glucose maintained at ~15 mM via insulin) and in vitro treatment of platelets with the specific Syk inhibitor BAY61-3606.
C
Comparator
Well-controlled diabetes (blood glucose maintained at ~8 mM via insulin) and healthy monkeys.
O
Outcome
Early downstream signalling events following engagement of glycoprotein (GP)VI, specifically platelet reactive oxygen species (ROS) generation and calcium mobilisation.surrogate

Poor glycaemic control exacerbates GPVI-dependent platelet hyperreactivity in a primate model of Type 1 diabetes, which can be mitigated by Syk inhibition, highlighting a potential novel antithrombotic target.

Cite This Study

Arthur et al. (2013) studied Type 1 diabetes. Poorly controlled diabetes and Syk inhibitor BAY61-3606 vs. Well-controlled diabetes and healthy monkeys was evaluated on Platelet reactive oxygen species (ROS) generation, calcium mobilisation, receptor surface expression, and immature platelet fraction. Poorly controlled diabetes (~15 mM glucose) exacerbated GPVI-dependent platelet ROS generation and calcium flux, which was reversed to healthy levels by the Syk inhibitor BAY61-3606.

synapsesocial.com/papers/6a6de80bac440176ef274fffhttps://doi.org/10.1155/2013/370212
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