The production and H-gene typing of isogenic resistant (IR) lines in the mouse is described. Thirty-eight lines reached a stage of development where significant information as to their genotype could be obtained; 26 of these were studied in some detail. Of the 38 original IR lines, 30 were shown to be distinguished from their inbred or “coisogenic” mate by a difference at the H-2 locus. One coisogenic pair, C3H and C3H.K, differs at H-1, another, C57BL/10 and B10.LP, at H-3. Of the 6 remaining lines, 1 probably had an H-1 difference, 3 were uncertain, and 2, still under test, may serve to define new loci. Two lines served to identify new H-2 alleles: D1.C is H-2c AKR.M is H-2m. The component test showed D1.C to have the D component, and AKR.M the K component. Linkage tests showed 7.1 ± 4.5 percent crossing over between H-1 and c, and 9.8 ± 6.4 percent crossing over between H-3 and A, both cases in heterozygous females. These values may be high. Evidence is presented that F1 hybrids, with the tumor donor as one parent, are less compatible with tumor grafts than the native strain. With one exception, the resistance shown by the hybrid was slight. It was not increased by immunization. The extent to which the synthesized IR lines and their inbred mates approach the true coisogenic state is discussed. Table 15 presents a summary of the current state of our information in regard to the H-2 locus.
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Snell Gd (1958) studied this question.