Human mast cells release extracellular vesicle-associated DNA.

Extracellular vesicles (EVs) carry multiple bioactive molecules, including proteins and nucleic acids. Cell-free, extracellular DNA has been reported to be present in cell cultures and in multiple body fluids, but its relative location in relation to EVs has not been described. This study demonstrates that DNase sensitive nucleic acids are present on the surface of EV isolates. Association of EV-DNA was revealed by increase of EV zeta potential and particle number upon DNase treatment. Additionally, Cells exposed to EVs with associated DNA, show the presence of cytoplasmic DNA traces intracellularly. In conclusion, we suggest that DNA can be associated to the surface of EVs, and can be taken up by recipient cells. DNA on EV surfaces may influence their function in recipient cells.